Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography
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چکیده
منابع مشابه
Simultaneous determination of caffeine, theobromine, and theophylline by high-performance liquid chromatography.
This work relates the development of an analytical methodology to simultaneously determine three methylxanthines (caffeine, theobromine, and theophylline) in beverages and urine samples based on reversed-phase high-performance liquid chromatography. Separation is made with a Bondesil C18 column using methanol-water-acetic acid or ethanol-water-acetic acid (20:75:5, v/v/v) as the mobile phase at...
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Two ion chromatographic methods have been proposed for the simultaneous determination of caffeine, theobromine and theophylline. The separations were based on isocratic elutions and the determinations on ultraviolet absorbance detections at 274 nm. The detection limits (signal-to-noise ratio 3:1) for the three compounds were all below sub-mg mlÿ1 level. The methods have been successfully applie...
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Background and objectives: High concentration of preservatives in food may result in gastrointestinal disturbances whereby some patients suffering from asthma, rhinitis, or urticaria. The aim of this study is the introduction and optimization a new method for simultaneous determination of four preservatives (SB, PS, MP, PP) in foodstuff by high performance liquid chromatography. Materi...
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A rapid and selective high-performance liquid chromatographic (HPLC) method is developed for the separation and determination of caffeine, theobromine, and theophylline. The chromatography is performed on a Zorbax Eclipse XDB-C8 column (4.6x150 mm i.d., 5-microm particle size) at 25 degrees C, with a mobile phase of water-THF (0.1% THF in water, pH 8)-acetonitrile (90:10, v/v). The flow rate is...
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Therapeutic Drug Monitoring (TDM) of first-line anti-tuberculosis (TB) drugs is a decisive tool, allowing the clinician to successfully treat TB patients. The objective of the study was to develop and optimize a simple, sensitive, and reliable high-performance liquid chromatography (HPLC) method for the simultaneous determination of isoniazid (INH), pyrazinamide (PZA), and rifampin (RIF) levels...
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ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 2002
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/40.1.45